Research Profile: On-line, real-time trypsin digestion in a nanoLC/MS system

Kinetics of Trypsin Digestion

The anomalies of enzyme action have frequently been accounted for by the assumption of an intermediate compound between the enzyme and the substrate. I t was shown, however, by Nelson and Hitchcock t that in the case of invertase the experiments could not be accurately calculated on this basis. The writer found with trypsin' that although the effect of varying the substrate concentration could ...

On-Line Learning of a Persian Spoken Dialogue System Using Real Training Data

The first spoken dialogue system developed for the Persian language is introduced. This is a ticket reservation system with Persian ASR and NLU modules. The focus of the paper is on learning the dialogue management module. In this work, real on-line training data are used during the learning process. For on-line learning, the effect of the variations of discount factor (g) on the learning speed...

On-Line Learning of a Persian Spoken Dialogue System Using Real Training Data

The first spoken dialogue system developed for the Persian language is introduced. This is a ticket reservation system with Persian ASR and NLU modules. The focus of the paper is on learning the dialogue management module. In this work, real on-line training data are used during the learning process. For on-line learning, the effect of the variations of discount factor (g) on the learning speed...

On-Line, Real-Time, Multiple-Speech Output System

The kinetics of trypsin digestion.

The present study of a typical proteolytic enzyme extends the quantitative investigations previously reported from this laboratory on the effects of pressure on protein systems in viva (1) and in vitro (2). This paper presents new data on the self-digestion of trypsin and the digestion of casein by trypsin; a forthcoming paper will discuss the effects of temperature and hydrostatic pressure on ...